Despite being a life-saving therapeutic approach in various diseases and conditions, parenteral nutrition has been associated with several complications, among which the liver injury. Different forms of liver injury have been reported in patients on long-term parenteral nutrition, including steatosis, steatohepatitis, cholestasis, fibrosis etc. In addition to risk factors related to patents’ specific condition, other risk factors linked to duration of parenteral nutrition, its composition, and caloric overload can also contribute to development of liver injury. 3R-imπACT project aims to evaluate the effects of parenteral nutrition on the liver, with particular focus on the mechanisms of hepatotoxicity. Given the ethical, scientific and financial concerns associated with animal testing, there is a strong need for using non-animal approaches together with mechanistic information in food safety assessment, thus complying with the 3R concept (replacement, reduction, refinement of animal testing). Therefore, the current project will make use of advanced, human-based and mechanistically anchored in vitro test battery, to be combined with exposure assessment in order to allow full animal-free and human-relevant safety evaluation of parenteral nutrition.
Mihajlovic, Milos, and Mathieu Vinken. “Mitochondria as the target of hepatotoxicity and drug-induced liver injury: molecular mechanisms and detection methods.” International Journal of Molecular Sciences 23.6 (2022): 3315.
Rodrigues, Bruna Dos Santos, et al. “Evaluation of Functional Candidate Biomarkers of Non-genotoxic Hepatocarcinogenicity in Human Liver Spheroid Co-cultures.” Archives of Toxicology (2023): 10.1007/s00204-023-03486-4
My Training and conferences
MSCA-COFUND imπACT workshops/training on research impact development and entrepreneurship, Vrije Universiteit Brussel, Brussels, Belgium (2022-2023)
My social media activity
LinkedIn: Milos Mihajlovic | LinkedIn